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mouse collagen type i elisa kit  (Cusabio)


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    Cusabio mouse collagen type i elisa kit
    Mouse Collagen Type I Elisa Kit, supplied by Cusabio, used in various techniques. Bioz Stars score: 93/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+collagen+type+i+elisa+kit/Mouse+Collagen+Type+%E2%85%A1%2CCol+%E2%85%A1+ELISA+Kit/us12569482-598-38-44
    Average 93 stars, based on 8 article reviews
    mouse collagen type i elisa kit - by Bioz Stars, 2026-09
    93/100 stars

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    Enzyme-linked Immunosorbent Assay:

    Article Title: The Biomimetics of Mg 2+ -Concentration-Resolved Microenvironment for Bone and Cartilage Repairing Materials Design
    Article Snippet: Chemicals and materials (and their purity and manufacture) in the experiment are listed as follows: Magnesium chloride hexahydrate (99%, Aladdin, Shanghai, China), Collagenase Type II (Sigma Aldrich, St. Louis, MO, USA), Trypsin (Sigma Aldrich, St. Louis, MO, USA), Phosphate Buffer Saline (PBS, Hyclone, Cytiva, Logan, UT, USA), Penicillin–streptomycin (Hyclone, Cytiva, Logan, UT, USA), Fetal Bovine Serum (FBS, Biological Industries, Israel), Dulbecco’s modified Eagle’s Medium (DMEM) of High glucose (HG, 4500 mg/dL) (DEME-HG; Hyclone, Cytiva, Logan, UT, USA), Dulbecco’s modified Eagle’s Medium/Nutrient Mixture F12 (DEME/F12; Hyclone, Cytiva, Logan, UT, USA), Cell counting kit-8 (CCK8, Solarbio, Beijing, China), Crystal violet (Solarbio, Beijing, China), Calcein-AM/Propidium Iodide (PI) live/dead cell staining kit (Solarbio, Beijing, China), 4′,6-diamidino-2-phenylindole (DAPI, Solarbio, Beijing, China), FITC-phalloidin (Solarbio, Beijing, China), Lipopolysaccharide (LPS, Solarbio, Beijing, China), L-Ascorbic acid (Sigma Aldrich, A4403, St. Louis, MO, USA), β-Glycerophosphate disodium salt hydrate (Sigma Aldrich, G9422, St. Louis, MO, USA), Dexamethasone (Sigma Aldrich, D4902, St. Louis, MO, USA), Alkaline Phosphatase (ALP) Assay kit (Beyotime Biotechnology, Shanghai, China), BCIP/NBT Alkaline Phosphatase (ALP) Assay Chromogenic Kit (Beyotime Biotechnology, Shanghai, China), Alizarin red (Solarbio, Beijing, China), Trizol reagent (Yeason Biotech, Shanghai, China), Hifair ® II First Strand cDNA Synthesis Kit (Yeason Biotech, Shanghai, China), Hieff ® qPCR SYBR Green Master Mix (Yeason Biotech, Shanghai, China). .. Rabbit Collagen Type II ELISA Kit (Cusabio, Wuhan, China), Rabbit Collagen Type X ELISA Kit (MyBioSource, San Diego, CA, USA), Rabbit Collagen Type I ELISA Kit (Cusabio, Wuhan, China), Mouse Collagen Type I ELISA Kit (Cusabio, Wuhan, China). ..

    Article Title: The miR-320a/PRDX3 Axis Alleviates the Oxidative Stress and Fibrotic Alterations in Fibroblasts in Thyroid Eye Disease.
    Article Snippet: The secreted Collagen I levels of OFs in culture medium were determined by the Human Collagen Type I ELISA Kit (CSB-E08082h; CUSABIO). .. The Collagen I level of in orbital tissues homogenates were determined by the mouse Collagen Type I ELISA Kit (CSB-E08083m; CUSABIO). .. The protein concentration of cells and tissue homogenates were determined by a protein assay BCA kit (Beyotime).

    Article Title: The miR-320a/PRDX3 Axis Alleviates the Oxidative Stress and Fibrotic Alterations in Fibroblasts in Thyroid Eye Disease
    Article Snippet: The secreted Collagen I levels of OFs in culture medium were determined by the Human Collagen Type I ELISA Kit (CSB-E08082h; CUSABIO). .. The Collagen I level of in orbital tissues homogenates were determined by the mouse Collagen Type I ELISA Kit (CSB-E08083m; CUSABIO). .. The protein concentration of cells and tissue homogenates were determined by a protein assay BCA kit (Beyotime).

    Article Title: Methods and compositions for treating idiopathic pulmonary fibrosis
    Article Snippet: The level of hydroxyproline in the lung tissues was measured using a Hydroxyproline Assay kit (Nanjing JianCheng Bioengineering Institute). .. The level of TGF-β1 in 100 μL BALF was determined using a mouse TGF-β1 ELISA kit (Beijing 4A Biotech Co., LTD), and the level of type I collagen (Col I) in 100 μL BALF was determined using a mouse Collagen Type I ELISA kit (CUSABIO BIOTEH). ..

    Article Title: Targeting the HSP47-collagen axis inhibits brain metastasis by reversing M2 microglial polarization and restoring anti-tumor immunity
    Article Snippet: True-Nuclear Transcription Factor Buffer Set , Biolegend , Cat# 424401. .. Mouse Collagen Type I ELISA Kit , CUSABIO , Cat# CSB-E08083m. .. Mouse PBMC Isolation Kit , IPHASE , Cat# 071E100.11.

    Article Title: Targeting the HSP47-collagen axis inhibits brain metastasis by reversing M2 microglial polarization and restoring anti-tumor immunity.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-HSP47 proteintech Cat# 10875-1-AP; RRID: AB_2301500 Anti-HSP47 Santa Cruz Cat# sc-5293; RRID: AB_627757 Anti-N-cadherin proteintech Cat# 22018-1-AP; RRID: AB_2813891 Anti-E-cadherin proteintech Cat# 20874-1-AP; RRID: AB_10697811 Anti-Snail proteintech Cat# 13099-1-AP; RRID: AB_2191756 Anti-Collagen I proteintech Cat# 14695-1-AP; RRID: AB_2082037 Anti-Collagen I proteintech Cat# 67288-1-Ig; RRID: AB_2882554 Anti-Collagen II proteintech Cat# 28459-1-AP; RRID: AB_2881147 Anti-Collagen III proteintech Cat# 22734-1-AP; RRID: AB_2879158 Anti-Collagen IV proteintech Cat# 19674-1-AP; RRID: AB_2878595 Anti-GFP-Tag Abclonal Cat# AE012; RRID: AB_2770402 Anti-Arg1 proteintech Cat# 16001-1-AP; RRID: AB_2289842 Anti-CD163 proteintech Cat# 16646-1-AP; RRID: AB_2756528 Anti-CD206 proteintech Cat# 18704-1-AP; RRID: AB_10597232 Anti- CD11c proteintech Cat# 17342-1-AP; RRID: AB_2129787 Anti-IBA1 proteintech Cat# 10904-1-AP; RRID: AB_2224377 Anti-CD8 Abcam Cat# ab217344; RRID: AB_2890649 Anti-CD8 Santa Cruz Cat# sc-7970; RRID: AB_627208 Anti-P65 CST Cat# 8242; RRID: AB_10859369 Anti-Phospho-P65 CST Cat# 3033; RRID: AB_331284 Anti-Tubulin Ray antibody Cat# RM2007; RRID: AB_2934267 CD11b-BV605 Biolegend Cat# 101257; RRID: AB_2565431 CD11c-APC Biolegend Cat# 117310; RRID: AB_313779 CD206-PE Biolegend Cat# 141706; RRID: AB_10895754 CD45-PE/cy7 Biolegend Cat# 103114; RRID: AB_312979 CD45-APC/cy7 BD Pharmingen Cat# 557659; RRID: AB_396774 CD3-PerCP/cy5.5 Biolegend Cat# 100218; RRID: AB_1595492 CD3-FITC Biolegend Cat# 100204; RRID: AB_312661 CD4-PE Biolegend Cat# 100408; RRID: AB_312693 CD8-APC/FireTM 750 Biolegend Cat# 100766; RRID: AB_2572113 CD8 PerCP/cy5.5 BD Pharmingen Cat# 551162; RRID: AB_394081 GZMB-BV421 Biolegend Cat# 396414; RRID: AB_2810603 TNF-a-BV650 Biolegend Cat# 506333; RRID: AB_2562450 IFN-g-BV510 Biolegend Cat# 505842; RRID: AB_2734494 Ki67-BV786 BD Pharmingen Cat# 563756; RRID: AB_2732007 CD69-AF700 Biolegend Cat# 104539; RRID: AB_2566304 CD25-BV605 BD Pharmingen Cat# 563061; RRID: AB_2737982 FOXP3-AF647 BD Pharmingen Cat# 560401; RRID: AB_1645201 Bacterial and virus strains EF1A-MCS-SV40-firefly_Luciferase-IRES-Puromycin Genechem https://www.genechem.com.cn/ pCLenti-EF1-Neo-CMV-HSP47-WPRE HanBio https://hanbio.net/ Tet-pLKO-neo YouBio http://www.youbio.cn/vector CMV-MCS-3flag-EF1-GFP-T2A-Blasticidin Genechem https://www.genechem.com.cn/ (Continued on next page) e1 Cell Reports Medicine 5, 101533, May 21, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER Biological samples Human primary breast cancer tissues and paired brain metastatic tissues Guangdong Provincial People’s Hospital of Southern Medical University (Guangzhou, China) N/A Human primary lung cancer tissues and paired brain metastatic tissues Guangdong Provincial People’s Hospital of Southern Medical University (Guangzhou, China) N/A Chemicals, peptides, and recombinant proteins Puromycin Thermo Fisher Scientific Cat# A1113803 Neomycin Solarbio Cat# IN0130 Blasticidin Selleck Cat# S7419 Col003 MedChemExpress Cat# HY-124817 TC-I 15 MedChemExpress Cat# HY-107588 InVivoMAb anti-mouse CD8a Selleck Cat# A2102 IgG2b isotype control-InVivo Selleck Cat# A2116 InVivoMAb anti-mouse PD-L1 (B7-H1) Bio X Cell Cat# BE0361; RRID: AB_2927503 PLX3397 MolDiets Cat# M18072603 Cultrex rCollagen I R&D Systems Cat# 3440-100-01 Collagenase 1 Worthington Cat# LS004197 ACK Lysis Buffer LEAGENE Cat# CS0001 Dnase 1 Solarbio Cat# D8071 DAPI Abcam Cat# ab104139 Citrate Sodium Buffer, pH 6.0 Solarbio Cat# C1010 Tris-EDTA buffer, pH 9.0 Elabscience Cat# E-IR-R104 Fetal Bovine Serum Gibco Cat# 10099141 Tween 20 Beyotime Cat# ST1727 Triton X-100 Beyotime Cat# ST797 RIPA buffer Beyotime Cat# P0013B Trizol reagent Invitrogen Cat# 15596026 D-Luciferin potassium salt Beyotime Cat# ST196 Lipofectamine 2000 reagent Invitrogen Cat# 11668019 Critical commercial assays RNeasy Mini Kit Qiagen Cat# 74104 PrimeScriptTM RT reagent Kit TAKARA Cat# RR037A CUT&Tag Kit YEASEN Cat# 12598ES12 True-Nuclear Transcription Factor Buffer Set Biolegend Cat# 424401 Mouse Collagen Type I ELISA Kit CUSABIO Cat# CSB-E08083m Mouse PBMC Isolation Kit IPHASE Cat# 071E100.11 Mouse CD8+ T cell Isolation Kit IPHASE Cat# 071E203.11 Deposited data RNA-seq of BV2 cells This paper GEO: GSE261941 Experimental models: Cell lines 4T1 Type Culture Collection of Chinese Academy of Sciences (Shanghai, China) N/A LLC1 Type Culture Collection of Chinese Academy of Sciences (Shanghai, China) N/A BV2 BeNa Culture Collection (Beijing, China) N/A 293FT BeNa Culture Collection (Beijing, China) N/A (Continued on next page) Cell Reports Medicine 5, 101533, May 21, 2024 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Organisms/strains BALB/C mice Guangdong Medical Laboratory Animal Center (Foshan, China) N/A C57BL/6 mice Guangdong Medical Laboratory Animal Center (Foshan, China) N/A Oligonucleotides Primer sequences are provided in the Table S1 This paper N/A Software and algorithms FlowJo BD Biosciences https://www.flowjo.com/ Prism, v8 GraphPad https://www.graphpad.com/ scientificsoftware/prism/ ImageJ NIH Image https://imagej.nih.gov/ij/index.html R (version 4.3.2) The R Project for Statistical Computing https://www.r-project.org/ R Studio PBC https://www.rstudio.com/ ComplexHeatmap (R package, version 2.16.0) N/A https://bioconductor.org/packages/ release/bioc/html/Complex Heatmap.html circlize (R package, version 0.4.15) N/A https://jokergoo.github.io/ circlize_book/book/ tidyverse (R package, version 2.0.0) N/A https://www.tidyverse.org/learn/ ggplot2 (R package, version 3.4.4) N/A https://ggplot2.tidyverse.org UMAP N/A https://arxiv.org/abs/1802.03426 ggvenn (R package, version 0.1.10) N/A https://CRAN.R-project.org/ package=ggvenn AnnotationDbi (R package, version 1.62.2) Bioconductor https://bioconductor.org/packages/ AnnotationDbi clusterProfiler (R package, version 4.8.3) Wu et al.39 https://yulab-smu.top/biomedical- knowledge-mining-book/ enrichplot (R package, version 1.20.3) Bioconductor https://bioconductor.org/packages/ release/bioc/html/enrichplot.html FastQC v0.11.9 N/A https://www.bioinformatics.babraham. ac.uk/projects/fastqc/ Trim galore v0.6.7 N/A https://www.bioinformatics.babraham. ac.uk/projects/trim_galore/ Hisat2 v2.2.1 Kim et al.41 http://daehwankimlab.github.io/hisat2/ DEseq2 (R package, version 1.40.2) Love et al.42 https://bioconductor.org/packages/ release/bioc/html/DESeq2.html infercnvpy v0.4.3 N/A https://infercnvpy.readthedocs.io/ en/latest/ Seurat (R package, version 5.0.1) N/A http://satijalab.org/seurat scanpy v1.9.3 N/A https://scanpy.readthedocs.io/ en/stable/ Other PVDF membrane Millipore Cat# IPVH00010 24-well plate Corning Cat# 3524 0.45 mm filter Sorfa Cat# 622120 70 mm cell filter ABCbio Cat# ABC60070 Transwell insert Corning Cat# 3422 e3 Cell Reports Medicine 5, 101533, May 21, 2024

    Article Title: Protective effects of a novel FS-Collagen hydrolysates against UV- and d -galactose-induced skin aging
    Article Snippet: The stained region was photographed using an Eclipse Ti-S inverted fluorescence microscope (Nikon, Japan), and the dermal collagen density was measured and analyzed. .. The content of collagen I (Mouse Collagen Type I ELISA Kit, CUSABIO), collagen III (Mouse Collagen Type III ELISA Kit, CUSABIO), hydroxyproline [Hydroxyproline (HYP) Content Assay Kit, BC0250, Solarbio] and hyaluronic acid (Hyaluronan Elisa Kit, SEKH-0509, Solarbio) were measured by the corresponding testing kits. .. The oxidation levels were measured by the Lipid Peroxidation MDA Assay Kit (S0131, Beyotime), Reactive Oxygen Species Assay Kit (S0033, Beyotime), Total Superoxide Dismutase Assay Kit with WST-8 (S0101, Beyotime), and Total Glutathione Peroxidase Assay Kit with NADPH (S0058, Beyotime), according to the manufacturer’s instructions.

    Article Title: Protective effects of a novel FS-Collagen hydrolysates against UV- and d-galactose-induced skin aging.
    Article Snippet: The stained region was photographed using an Eclipse Ti-S inverted fluorescence microscope (Nikon, Japan), and the dermal collagen density was measured and analyzed. .. The content of collagen I (Mouse Collagen Type I ELISA Kit, CUSABIO), collagen III (Mouse Collagen Type III ELISA Kit, CUSABIO), hydroxyproline [Hydroxyproline (HYP) Content Assay Kit, BC0250, Solarbio] and hyaluronic acid (Hyaluronan Elisa Kit, SEKH-0509, Solarbio) were measured by the corresponding testing kits. .. The oxidation levels were measured by the Lipid Peroxidation MDA Assay Kit (S0131, Beyotime), Reactive Oxygen Species Assay Kit (S0033, Beyotime), Total Superoxide Dismutase Assay Kit with WST-8 (S0101, Beyotime), and Total Glutathione Peroxidase Assay Kit with NADPH (S0058, Beyotime), according to the manufacturer’s instructions.

    Recombinant:

    Article Title: Targeting the HSP47-collagen axis inhibits brain metastasis by reversing M2 microglial polarization and restoring anti-tumor immunity.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-HSP47 proteintech Cat# 10875-1-AP; RRID: AB_2301500 Anti-HSP47 Santa Cruz Cat# sc-5293; RRID: AB_627757 Anti-N-cadherin proteintech Cat# 22018-1-AP; RRID: AB_2813891 Anti-E-cadherin proteintech Cat# 20874-1-AP; RRID: AB_10697811 Anti-Snail proteintech Cat# 13099-1-AP; RRID: AB_2191756 Anti-Collagen I proteintech Cat# 14695-1-AP; RRID: AB_2082037 Anti-Collagen I proteintech Cat# 67288-1-Ig; RRID: AB_2882554 Anti-Collagen II proteintech Cat# 28459-1-AP; RRID: AB_2881147 Anti-Collagen III proteintech Cat# 22734-1-AP; RRID: AB_2879158 Anti-Collagen IV proteintech Cat# 19674-1-AP; RRID: AB_2878595 Anti-GFP-Tag Abclonal Cat# AE012; RRID: AB_2770402 Anti-Arg1 proteintech Cat# 16001-1-AP; RRID: AB_2289842 Anti-CD163 proteintech Cat# 16646-1-AP; RRID: AB_2756528 Anti-CD206 proteintech Cat# 18704-1-AP; RRID: AB_10597232 Anti- CD11c proteintech Cat# 17342-1-AP; RRID: AB_2129787 Anti-IBA1 proteintech Cat# 10904-1-AP; RRID: AB_2224377 Anti-CD8 Abcam Cat# ab217344; RRID: AB_2890649 Anti-CD8 Santa Cruz Cat# sc-7970; RRID: AB_627208 Anti-P65 CST Cat# 8242; RRID: AB_10859369 Anti-Phospho-P65 CST Cat# 3033; RRID: AB_331284 Anti-Tubulin Ray antibody Cat# RM2007; RRID: AB_2934267 CD11b-BV605 Biolegend Cat# 101257; RRID: AB_2565431 CD11c-APC Biolegend Cat# 117310; RRID: AB_313779 CD206-PE Biolegend Cat# 141706; RRID: AB_10895754 CD45-PE/cy7 Biolegend Cat# 103114; RRID: AB_312979 CD45-APC/cy7 BD Pharmingen Cat# 557659; RRID: AB_396774 CD3-PerCP/cy5.5 Biolegend Cat# 100218; RRID: AB_1595492 CD3-FITC Biolegend Cat# 100204; RRID: AB_312661 CD4-PE Biolegend Cat# 100408; RRID: AB_312693 CD8-APC/FireTM 750 Biolegend Cat# 100766; RRID: AB_2572113 CD8 PerCP/cy5.5 BD Pharmingen Cat# 551162; RRID: AB_394081 GZMB-BV421 Biolegend Cat# 396414; RRID: AB_2810603 TNF-a-BV650 Biolegend Cat# 506333; RRID: AB_2562450 IFN-g-BV510 Biolegend Cat# 505842; RRID: AB_2734494 Ki67-BV786 BD Pharmingen Cat# 563756; RRID: AB_2732007 CD69-AF700 Biolegend Cat# 104539; RRID: AB_2566304 CD25-BV605 BD Pharmingen Cat# 563061; RRID: AB_2737982 FOXP3-AF647 BD Pharmingen Cat# 560401; RRID: AB_1645201 Bacterial and virus strains EF1A-MCS-SV40-firefly_Luciferase-IRES-Puromycin Genechem https://www.genechem.com.cn/ pCLenti-EF1-Neo-CMV-HSP47-WPRE HanBio https://hanbio.net/ Tet-pLKO-neo YouBio http://www.youbio.cn/vector CMV-MCS-3flag-EF1-GFP-T2A-Blasticidin Genechem https://www.genechem.com.cn/ (Continued on next page) e1 Cell Reports Medicine 5, 101533, May 21, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER Biological samples Human primary breast cancer tissues and paired brain metastatic tissues Guangdong Provincial People’s Hospital of Southern Medical University (Guangzhou, China) N/A Human primary lung cancer tissues and paired brain metastatic tissues Guangdong Provincial People’s Hospital of Southern Medical University (Guangzhou, China) N/A Chemicals, peptides, and recombinant proteins Puromycin Thermo Fisher Scientific Cat# A1113803 Neomycin Solarbio Cat# IN0130 Blasticidin Selleck Cat# S7419 Col003 MedChemExpress Cat# HY-124817 TC-I 15 MedChemExpress Cat# HY-107588 InVivoMAb anti-mouse CD8a Selleck Cat# A2102 IgG2b isotype control-InVivo Selleck Cat# A2116 InVivoMAb anti-mouse PD-L1 (B7-H1) Bio X Cell Cat# BE0361; RRID: AB_2927503 PLX3397 MolDiets Cat# M18072603 Cultrex rCollagen I R&D Systems Cat# 3440-100-01 Collagenase 1 Worthington Cat# LS004197 ACK Lysis Buffer LEAGENE Cat# CS0001 Dnase 1 Solarbio Cat# D8071 DAPI Abcam Cat# ab104139 Citrate Sodium Buffer, pH 6.0 Solarbio Cat# C1010 Tris-EDTA buffer, pH 9.0 Elabscience Cat# E-IR-R104 Fetal Bovine Serum Gibco Cat# 10099141 Tween 20 Beyotime Cat# ST1727 Triton X-100 Beyotime Cat# ST797 RIPA buffer Beyotime Cat# P0013B Trizol reagent Invitrogen Cat# 15596026 D-Luciferin potassium salt Beyotime Cat# ST196 Lipofectamine 2000 reagent Invitrogen Cat# 11668019 Critical commercial assays RNeasy Mini Kit Qiagen Cat# 74104 PrimeScriptTM RT reagent Kit TAKARA Cat# RR037A CUT&Tag Kit YEASEN Cat# 12598ES12 True-Nuclear Transcription Factor Buffer Set Biolegend Cat# 424401 Mouse Collagen Type I ELISA Kit CUSABIO Cat# CSB-E08083m Mouse PBMC Isolation Kit IPHASE Cat# 071E100.11 Mouse CD8+ T cell Isolation Kit IPHASE Cat# 071E203.11 Deposited data RNA-seq of BV2 cells This paper GEO: GSE261941 Experimental models: Cell lines 4T1 Type Culture Collection of Chinese Academy of Sciences (Shanghai, China) N/A LLC1 Type Culture Collection of Chinese Academy of Sciences (Shanghai, China) N/A BV2 BeNa Culture Collection (Beijing, China) N/A 293FT BeNa Culture Collection (Beijing, China) N/A (Continued on next page) Cell Reports Medicine 5, 101533, May 21, 2024 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Organisms/strains BALB/C mice Guangdong Medical Laboratory Animal Center (Foshan, China) N/A C57BL/6 mice Guangdong Medical Laboratory Animal Center (Foshan, China) N/A Oligonucleotides Primer sequences are provided in the Table S1 This paper N/A Software and algorithms FlowJo BD Biosciences https://www.flowjo.com/ Prism, v8 GraphPad https://www.graphpad.com/ scientificsoftware/prism/ ImageJ NIH Image https://imagej.nih.gov/ij/index.html R (version 4.3.2) The R Project for Statistical Computing https://www.r-project.org/ R Studio PBC https://www.rstudio.com/ ComplexHeatmap (R package, version 2.16.0) N/A https://bioconductor.org/packages/ release/bioc/html/Complex Heatmap.html circlize (R package, version 0.4.15) N/A https://jokergoo.github.io/ circlize_book/book/ tidyverse (R package, version 2.0.0) N/A https://www.tidyverse.org/learn/ ggplot2 (R package, version 3.4.4) N/A https://ggplot2.tidyverse.org UMAP N/A https://arxiv.org/abs/1802.03426 ggvenn (R package, version 0.1.10) N/A https://CRAN.R-project.org/ package=ggvenn AnnotationDbi (R package, version 1.62.2) Bioconductor https://bioconductor.org/packages/ AnnotationDbi clusterProfiler (R package, version 4.8.3) Wu et al.39 https://yulab-smu.top/biomedical- knowledge-mining-book/ enrichplot (R package, version 1.20.3) Bioconductor https://bioconductor.org/packages/ release/bioc/html/enrichplot.html FastQC v0.11.9 N/A https://www.bioinformatics.babraham. ac.uk/projects/fastqc/ Trim galore v0.6.7 N/A https://www.bioinformatics.babraham. ac.uk/projects/trim_galore/ Hisat2 v2.2.1 Kim et al.41 http://daehwankimlab.github.io/hisat2/ DEseq2 (R package, version 1.40.2) Love et al.42 https://bioconductor.org/packages/ release/bioc/html/DESeq2.html infercnvpy v0.4.3 N/A https://infercnvpy.readthedocs.io/ en/latest/ Seurat (R package, version 5.0.1) N/A http://satijalab.org/seurat scanpy v1.9.3 N/A https://scanpy.readthedocs.io/ en/stable/ Other PVDF membrane Millipore Cat# IPVH00010 24-well plate Corning Cat# 3524 0.45 mm filter Sorfa Cat# 622120 70 mm cell filter ABCbio Cat# ABC60070 Transwell insert Corning Cat# 3422 e3 Cell Reports Medicine 5, 101533, May 21, 2024

    Control:

    Article Title: Targeting the HSP47-collagen axis inhibits brain metastasis by reversing M2 microglial polarization and restoring anti-tumor immunity.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-HSP47 proteintech Cat# 10875-1-AP; RRID: AB_2301500 Anti-HSP47 Santa Cruz Cat# sc-5293; RRID: AB_627757 Anti-N-cadherin proteintech Cat# 22018-1-AP; RRID: AB_2813891 Anti-E-cadherin proteintech Cat# 20874-1-AP; RRID: AB_10697811 Anti-Snail proteintech Cat# 13099-1-AP; RRID: AB_2191756 Anti-Collagen I proteintech Cat# 14695-1-AP; RRID: AB_2082037 Anti-Collagen I proteintech Cat# 67288-1-Ig; RRID: AB_2882554 Anti-Collagen II proteintech Cat# 28459-1-AP; RRID: AB_2881147 Anti-Collagen III proteintech Cat# 22734-1-AP; RRID: AB_2879158 Anti-Collagen IV proteintech Cat# 19674-1-AP; RRID: AB_2878595 Anti-GFP-Tag Abclonal Cat# AE012; RRID: AB_2770402 Anti-Arg1 proteintech Cat# 16001-1-AP; RRID: AB_2289842 Anti-CD163 proteintech Cat# 16646-1-AP; RRID: AB_2756528 Anti-CD206 proteintech Cat# 18704-1-AP; RRID: AB_10597232 Anti- CD11c proteintech Cat# 17342-1-AP; RRID: AB_2129787 Anti-IBA1 proteintech Cat# 10904-1-AP; RRID: AB_2224377 Anti-CD8 Abcam Cat# ab217344; RRID: AB_2890649 Anti-CD8 Santa Cruz Cat# sc-7970; RRID: AB_627208 Anti-P65 CST Cat# 8242; RRID: AB_10859369 Anti-Phospho-P65 CST Cat# 3033; RRID: AB_331284 Anti-Tubulin Ray antibody Cat# RM2007; RRID: AB_2934267 CD11b-BV605 Biolegend Cat# 101257; RRID: AB_2565431 CD11c-APC Biolegend Cat# 117310; RRID: AB_313779 CD206-PE Biolegend Cat# 141706; RRID: AB_10895754 CD45-PE/cy7 Biolegend Cat# 103114; RRID: AB_312979 CD45-APC/cy7 BD Pharmingen Cat# 557659; RRID: AB_396774 CD3-PerCP/cy5.5 Biolegend Cat# 100218; RRID: AB_1595492 CD3-FITC Biolegend Cat# 100204; RRID: AB_312661 CD4-PE Biolegend Cat# 100408; RRID: AB_312693 CD8-APC/FireTM 750 Biolegend Cat# 100766; RRID: AB_2572113 CD8 PerCP/cy5.5 BD Pharmingen Cat# 551162; RRID: AB_394081 GZMB-BV421 Biolegend Cat# 396414; RRID: AB_2810603 TNF-a-BV650 Biolegend Cat# 506333; RRID: AB_2562450 IFN-g-BV510 Biolegend Cat# 505842; RRID: AB_2734494 Ki67-BV786 BD Pharmingen Cat# 563756; RRID: AB_2732007 CD69-AF700 Biolegend Cat# 104539; RRID: AB_2566304 CD25-BV605 BD Pharmingen Cat# 563061; RRID: AB_2737982 FOXP3-AF647 BD Pharmingen Cat# 560401; RRID: AB_1645201 Bacterial and virus strains EF1A-MCS-SV40-firefly_Luciferase-IRES-Puromycin Genechem https://www.genechem.com.cn/ pCLenti-EF1-Neo-CMV-HSP47-WPRE HanBio https://hanbio.net/ Tet-pLKO-neo YouBio http://www.youbio.cn/vector CMV-MCS-3flag-EF1-GFP-T2A-Blasticidin Genechem https://www.genechem.com.cn/ (Continued on next page) e1 Cell Reports Medicine 5, 101533, May 21, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER Biological samples Human primary breast cancer tissues and paired brain metastatic tissues Guangdong Provincial People’s Hospital of Southern Medical University (Guangzhou, China) N/A Human primary lung cancer tissues and paired brain metastatic tissues Guangdong Provincial People’s Hospital of Southern Medical University (Guangzhou, China) N/A Chemicals, peptides, and recombinant proteins Puromycin Thermo Fisher Scientific Cat# A1113803 Neomycin Solarbio Cat# IN0130 Blasticidin Selleck Cat# S7419 Col003 MedChemExpress Cat# HY-124817 TC-I 15 MedChemExpress Cat# HY-107588 InVivoMAb anti-mouse CD8a Selleck Cat# A2102 IgG2b isotype control-InVivo Selleck Cat# A2116 InVivoMAb anti-mouse PD-L1 (B7-H1) Bio X Cell Cat# BE0361; RRID: AB_2927503 PLX3397 MolDiets Cat# M18072603 Cultrex rCollagen I R&D Systems Cat# 3440-100-01 Collagenase 1 Worthington Cat# LS004197 ACK Lysis Buffer LEAGENE Cat# CS0001 Dnase 1 Solarbio Cat# D8071 DAPI Abcam Cat# ab104139 Citrate Sodium Buffer, pH 6.0 Solarbio Cat# C1010 Tris-EDTA buffer, pH 9.0 Elabscience Cat# E-IR-R104 Fetal Bovine Serum Gibco Cat# 10099141 Tween 20 Beyotime Cat# ST1727 Triton X-100 Beyotime Cat# ST797 RIPA buffer Beyotime Cat# P0013B Trizol reagent Invitrogen Cat# 15596026 D-Luciferin potassium salt Beyotime Cat# ST196 Lipofectamine 2000 reagent Invitrogen Cat# 11668019 Critical commercial assays RNeasy Mini Kit Qiagen Cat# 74104 PrimeScriptTM RT reagent Kit TAKARA Cat# RR037A CUT&Tag Kit YEASEN Cat# 12598ES12 True-Nuclear Transcription Factor Buffer Set Biolegend Cat# 424401 Mouse Collagen Type I ELISA Kit CUSABIO Cat# CSB-E08083m Mouse PBMC Isolation Kit IPHASE Cat# 071E100.11 Mouse CD8+ T cell Isolation Kit IPHASE Cat# 071E203.11 Deposited data RNA-seq of BV2 cells This paper GEO: GSE261941 Experimental models: Cell lines 4T1 Type Culture Collection of Chinese Academy of Sciences (Shanghai, China) N/A LLC1 Type Culture Collection of Chinese Academy of Sciences (Shanghai, China) N/A BV2 BeNa Culture Collection (Beijing, China) N/A 293FT BeNa Culture Collection (Beijing, China) N/A (Continued on next page) Cell Reports Medicine 5, 101533, May 21, 2024 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Organisms/strains BALB/C mice Guangdong Medical Laboratory Animal Center (Foshan, China) N/A C57BL/6 mice Guangdong Medical Laboratory Animal Center (Foshan, China) N/A Oligonucleotides Primer sequences are provided in the Table S1 This paper N/A Software and algorithms FlowJo BD Biosciences https://www.flowjo.com/ Prism, v8 GraphPad https://www.graphpad.com/ scientificsoftware/prism/ ImageJ NIH Image https://imagej.nih.gov/ij/index.html R (version 4.3.2) The R Project for Statistical Computing https://www.r-project.org/ R Studio PBC https://www.rstudio.com/ ComplexHeatmap (R package, version 2.16.0) N/A https://bioconductor.org/packages/ release/bioc/html/Complex Heatmap.html circlize (R package, version 0.4.15) N/A https://jokergoo.github.io/ circlize_book/book/ tidyverse (R package, version 2.0.0) N/A https://www.tidyverse.org/learn/ ggplot2 (R package, version 3.4.4) N/A https://ggplot2.tidyverse.org UMAP N/A https://arxiv.org/abs/1802.03426 ggvenn (R package, version 0.1.10) N/A https://CRAN.R-project.org/ package=ggvenn AnnotationDbi (R package, version 1.62.2) Bioconductor https://bioconductor.org/packages/ AnnotationDbi clusterProfiler (R package, version 4.8.3) Wu et al.39 https://yulab-smu.top/biomedical- knowledge-mining-book/ enrichplot (R package, version 1.20.3) Bioconductor https://bioconductor.org/packages/ release/bioc/html/enrichplot.html FastQC v0.11.9 N/A https://www.bioinformatics.babraham. ac.uk/projects/fastqc/ Trim galore v0.6.7 N/A https://www.bioinformatics.babraham. ac.uk/projects/trim_galore/ Hisat2 v2.2.1 Kim et al.41 http://daehwankimlab.github.io/hisat2/ DEseq2 (R package, version 1.40.2) Love et al.42 https://bioconductor.org/packages/ release/bioc/html/DESeq2.html infercnvpy v0.4.3 N/A https://infercnvpy.readthedocs.io/ en/latest/ Seurat (R package, version 5.0.1) N/A http://satijalab.org/seurat scanpy v1.9.3 N/A https://scanpy.readthedocs.io/ en/stable/ Other PVDF membrane Millipore Cat# IPVH00010 24-well plate Corning Cat# 3524 0.45 mm filter Sorfa Cat# 622120 70 mm cell filter ABCbio Cat# ABC60070 Transwell insert Corning Cat# 3422 e3 Cell Reports Medicine 5, 101533, May 21, 2024

    Lysis:

    Article Title: Targeting the HSP47-collagen axis inhibits brain metastasis by reversing M2 microglial polarization and restoring anti-tumor immunity.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-HSP47 proteintech Cat# 10875-1-AP; RRID: AB_2301500 Anti-HSP47 Santa Cruz Cat# sc-5293; RRID: AB_627757 Anti-N-cadherin proteintech Cat# 22018-1-AP; RRID: AB_2813891 Anti-E-cadherin proteintech Cat# 20874-1-AP; RRID: AB_10697811 Anti-Snail proteintech Cat# 13099-1-AP; RRID: AB_2191756 Anti-Collagen I proteintech Cat# 14695-1-AP; RRID: AB_2082037 Anti-Collagen I proteintech Cat# 67288-1-Ig; RRID: AB_2882554 Anti-Collagen II proteintech Cat# 28459-1-AP; RRID: AB_2881147 Anti-Collagen III proteintech Cat# 22734-1-AP; RRID: AB_2879158 Anti-Collagen IV proteintech Cat# 19674-1-AP; RRID: AB_2878595 Anti-GFP-Tag Abclonal Cat# AE012; RRID: AB_2770402 Anti-Arg1 proteintech Cat# 16001-1-AP; RRID: AB_2289842 Anti-CD163 proteintech Cat# 16646-1-AP; RRID: AB_2756528 Anti-CD206 proteintech Cat# 18704-1-AP; RRID: AB_10597232 Anti- CD11c proteintech Cat# 17342-1-AP; RRID: AB_2129787 Anti-IBA1 proteintech Cat# 10904-1-AP; RRID: AB_2224377 Anti-CD8 Abcam Cat# ab217344; RRID: AB_2890649 Anti-CD8 Santa Cruz Cat# sc-7970; RRID: AB_627208 Anti-P65 CST Cat# 8242; RRID: AB_10859369 Anti-Phospho-P65 CST Cat# 3033; RRID: AB_331284 Anti-Tubulin Ray antibody Cat# RM2007; RRID: AB_2934267 CD11b-BV605 Biolegend Cat# 101257; RRID: AB_2565431 CD11c-APC Biolegend Cat# 117310; RRID: AB_313779 CD206-PE Biolegend Cat# 141706; RRID: AB_10895754 CD45-PE/cy7 Biolegend Cat# 103114; RRID: AB_312979 CD45-APC/cy7 BD Pharmingen Cat# 557659; RRID: AB_396774 CD3-PerCP/cy5.5 Biolegend Cat# 100218; RRID: AB_1595492 CD3-FITC Biolegend Cat# 100204; RRID: AB_312661 CD4-PE Biolegend Cat# 100408; RRID: AB_312693 CD8-APC/FireTM 750 Biolegend Cat# 100766; RRID: AB_2572113 CD8 PerCP/cy5.5 BD Pharmingen Cat# 551162; RRID: AB_394081 GZMB-BV421 Biolegend Cat# 396414; RRID: AB_2810603 TNF-a-BV650 Biolegend Cat# 506333; RRID: AB_2562450 IFN-g-BV510 Biolegend Cat# 505842; RRID: AB_2734494 Ki67-BV786 BD Pharmingen Cat# 563756; RRID: AB_2732007 CD69-AF700 Biolegend Cat# 104539; RRID: AB_2566304 CD25-BV605 BD Pharmingen Cat# 563061; RRID: AB_2737982 FOXP3-AF647 BD Pharmingen Cat# 560401; RRID: AB_1645201 Bacterial and virus strains EF1A-MCS-SV40-firefly_Luciferase-IRES-Puromycin Genechem https://www.genechem.com.cn/ pCLenti-EF1-Neo-CMV-HSP47-WPRE HanBio https://hanbio.net/ Tet-pLKO-neo YouBio http://www.youbio.cn/vector CMV-MCS-3flag-EF1-GFP-T2A-Blasticidin Genechem https://www.genechem.com.cn/ (Continued on next page) e1 Cell Reports Medicine 5, 101533, May 21, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER Biological samples Human primary breast cancer tissues and paired brain metastatic tissues Guangdong Provincial People’s Hospital of Southern Medical University (Guangzhou, China) N/A Human primary lung cancer tissues and paired brain metastatic tissues Guangdong Provincial People’s Hospital of Southern Medical University (Guangzhou, China) N/A Chemicals, peptides, and recombinant proteins Puromycin Thermo Fisher Scientific Cat# A1113803 Neomycin Solarbio Cat# IN0130 Blasticidin Selleck Cat# S7419 Col003 MedChemExpress Cat# HY-124817 TC-I 15 MedChemExpress Cat# HY-107588 InVivoMAb anti-mouse CD8a Selleck Cat# A2102 IgG2b isotype control-InVivo Selleck Cat# A2116 InVivoMAb anti-mouse PD-L1 (B7-H1) Bio X Cell Cat# BE0361; RRID: AB_2927503 PLX3397 MolDiets Cat# M18072603 Cultrex rCollagen I R&D Systems Cat# 3440-100-01 Collagenase 1 Worthington Cat# LS004197 ACK Lysis Buffer LEAGENE Cat# CS0001 Dnase 1 Solarbio Cat# D8071 DAPI Abcam Cat# ab104139 Citrate Sodium Buffer, pH 6.0 Solarbio Cat# C1010 Tris-EDTA buffer, pH 9.0 Elabscience Cat# E-IR-R104 Fetal Bovine Serum Gibco Cat# 10099141 Tween 20 Beyotime Cat# ST1727 Triton X-100 Beyotime Cat# ST797 RIPA buffer Beyotime Cat# P0013B Trizol reagent Invitrogen Cat# 15596026 D-Luciferin potassium salt Beyotime Cat# ST196 Lipofectamine 2000 reagent Invitrogen Cat# 11668019 Critical commercial assays RNeasy Mini Kit Qiagen Cat# 74104 PrimeScriptTM RT reagent Kit TAKARA Cat# RR037A CUT&Tag Kit YEASEN Cat# 12598ES12 True-Nuclear Transcription Factor Buffer Set Biolegend Cat# 424401 Mouse Collagen Type I ELISA Kit CUSABIO Cat# CSB-E08083m Mouse PBMC Isolation Kit IPHASE Cat# 071E100.11 Mouse CD8+ T cell Isolation Kit IPHASE Cat# 071E203.11 Deposited data RNA-seq of BV2 cells This paper GEO: GSE261941 Experimental models: Cell lines 4T1 Type Culture Collection of Chinese Academy of Sciences (Shanghai, China) N/A LLC1 Type Culture Collection of Chinese Academy of Sciences (Shanghai, China) N/A BV2 BeNa Culture Collection (Beijing, China) N/A 293FT BeNa Culture Collection (Beijing, China) N/A (Continued on next page) Cell Reports Medicine 5, 101533, May 21, 2024 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Organisms/strains BALB/C mice Guangdong Medical Laboratory Animal Center (Foshan, China) N/A C57BL/6 mice Guangdong Medical Laboratory Animal Center (Foshan, China) N/A Oligonucleotides Primer sequences are provided in the Table S1 This paper N/A Software and algorithms FlowJo BD Biosciences https://www.flowjo.com/ Prism, v8 GraphPad https://www.graphpad.com/ scientificsoftware/prism/ ImageJ NIH Image https://imagej.nih.gov/ij/index.html R (version 4.3.2) The R Project for Statistical Computing https://www.r-project.org/ R Studio PBC https://www.rstudio.com/ ComplexHeatmap (R package, version 2.16.0) N/A https://bioconductor.org/packages/ release/bioc/html/Complex Heatmap.html circlize (R package, version 0.4.15) N/A https://jokergoo.github.io/ circlize_book/book/ tidyverse (R package, version 2.0.0) N/A https://www.tidyverse.org/learn/ ggplot2 (R package, version 3.4.4) N/A https://ggplot2.tidyverse.org UMAP N/A https://arxiv.org/abs/1802.03426 ggvenn (R package, version 0.1.10) N/A https://CRAN.R-project.org/ package=ggvenn AnnotationDbi (R package, version 1.62.2) Bioconductor https://bioconductor.org/packages/ AnnotationDbi clusterProfiler (R package, version 4.8.3) Wu et al.39 https://yulab-smu.top/biomedical- knowledge-mining-book/ enrichplot (R package, version 1.20.3) Bioconductor https://bioconductor.org/packages/ release/bioc/html/enrichplot.html FastQC v0.11.9 N/A https://www.bioinformatics.babraham. ac.uk/projects/fastqc/ Trim galore v0.6.7 N/A https://www.bioinformatics.babraham. ac.uk/projects/trim_galore/ Hisat2 v2.2.1 Kim et al.41 http://daehwankimlab.github.io/hisat2/ DEseq2 (R package, version 1.40.2) Love et al.42 https://bioconductor.org/packages/ release/bioc/html/DESeq2.html infercnvpy v0.4.3 N/A https://infercnvpy.readthedocs.io/ en/latest/ Seurat (R package, version 5.0.1) N/A http://satijalab.org/seurat scanpy v1.9.3 N/A https://scanpy.readthedocs.io/ en/stable/ Other PVDF membrane Millipore Cat# IPVH00010 24-well plate Corning Cat# 3524 0.45 mm filter Sorfa Cat# 622120 70 mm cell filter ABCbio Cat# ABC60070 Transwell insert Corning Cat# 3422 e3 Cell Reports Medicine 5, 101533, May 21, 2024

    Isolation:

    Article Title: Targeting the HSP47-collagen axis inhibits brain metastasis by reversing M2 microglial polarization and restoring anti-tumor immunity.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-HSP47 proteintech Cat# 10875-1-AP; RRID: AB_2301500 Anti-HSP47 Santa Cruz Cat# sc-5293; RRID: AB_627757 Anti-N-cadherin proteintech Cat# 22018-1-AP; RRID: AB_2813891 Anti-E-cadherin proteintech Cat# 20874-1-AP; RRID: AB_10697811 Anti-Snail proteintech Cat# 13099-1-AP; RRID: AB_2191756 Anti-Collagen I proteintech Cat# 14695-1-AP; RRID: AB_2082037 Anti-Collagen I proteintech Cat# 67288-1-Ig; RRID: AB_2882554 Anti-Collagen II proteintech Cat# 28459-1-AP; RRID: AB_2881147 Anti-Collagen III proteintech Cat# 22734-1-AP; RRID: AB_2879158 Anti-Collagen IV proteintech Cat# 19674-1-AP; RRID: AB_2878595 Anti-GFP-Tag Abclonal Cat# AE012; RRID: AB_2770402 Anti-Arg1 proteintech Cat# 16001-1-AP; RRID: AB_2289842 Anti-CD163 proteintech Cat# 16646-1-AP; RRID: AB_2756528 Anti-CD206 proteintech Cat# 18704-1-AP; RRID: AB_10597232 Anti- CD11c proteintech Cat# 17342-1-AP; RRID: AB_2129787 Anti-IBA1 proteintech Cat# 10904-1-AP; RRID: AB_2224377 Anti-CD8 Abcam Cat# ab217344; RRID: AB_2890649 Anti-CD8 Santa Cruz Cat# sc-7970; RRID: AB_627208 Anti-P65 CST Cat# 8242; RRID: AB_10859369 Anti-Phospho-P65 CST Cat# 3033; RRID: AB_331284 Anti-Tubulin Ray antibody Cat# RM2007; RRID: AB_2934267 CD11b-BV605 Biolegend Cat# 101257; RRID: AB_2565431 CD11c-APC Biolegend Cat# 117310; RRID: AB_313779 CD206-PE Biolegend Cat# 141706; RRID: AB_10895754 CD45-PE/cy7 Biolegend Cat# 103114; RRID: AB_312979 CD45-APC/cy7 BD Pharmingen Cat# 557659; RRID: AB_396774 CD3-PerCP/cy5.5 Biolegend Cat# 100218; RRID: AB_1595492 CD3-FITC Biolegend Cat# 100204; RRID: AB_312661 CD4-PE Biolegend Cat# 100408; RRID: AB_312693 CD8-APC/FireTM 750 Biolegend Cat# 100766; RRID: AB_2572113 CD8 PerCP/cy5.5 BD Pharmingen Cat# 551162; RRID: AB_394081 GZMB-BV421 Biolegend Cat# 396414; RRID: AB_2810603 TNF-a-BV650 Biolegend Cat# 506333; RRID: AB_2562450 IFN-g-BV510 Biolegend Cat# 505842; RRID: AB_2734494 Ki67-BV786 BD Pharmingen Cat# 563756; RRID: AB_2732007 CD69-AF700 Biolegend Cat# 104539; RRID: AB_2566304 CD25-BV605 BD Pharmingen Cat# 563061; RRID: AB_2737982 FOXP3-AF647 BD Pharmingen Cat# 560401; RRID: AB_1645201 Bacterial and virus strains EF1A-MCS-SV40-firefly_Luciferase-IRES-Puromycin Genechem https://www.genechem.com.cn/ pCLenti-EF1-Neo-CMV-HSP47-WPRE HanBio https://hanbio.net/ Tet-pLKO-neo YouBio http://www.youbio.cn/vector CMV-MCS-3flag-EF1-GFP-T2A-Blasticidin Genechem https://www.genechem.com.cn/ (Continued on next page) e1 Cell Reports Medicine 5, 101533, May 21, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER Biological samples Human primary breast cancer tissues and paired brain metastatic tissues Guangdong Provincial People’s Hospital of Southern Medical University (Guangzhou, China) N/A Human primary lung cancer tissues and paired brain metastatic tissues Guangdong Provincial People’s Hospital of Southern Medical University (Guangzhou, China) N/A Chemicals, peptides, and recombinant proteins Puromycin Thermo Fisher Scientific Cat# A1113803 Neomycin Solarbio Cat# IN0130 Blasticidin Selleck Cat# S7419 Col003 MedChemExpress Cat# HY-124817 TC-I 15 MedChemExpress Cat# HY-107588 InVivoMAb anti-mouse CD8a Selleck Cat# A2102 IgG2b isotype control-InVivo Selleck Cat# A2116 InVivoMAb anti-mouse PD-L1 (B7-H1) Bio X Cell Cat# BE0361; RRID: AB_2927503 PLX3397 MolDiets Cat# M18072603 Cultrex rCollagen I R&D Systems Cat# 3440-100-01 Collagenase 1 Worthington Cat# LS004197 ACK Lysis Buffer LEAGENE Cat# CS0001 Dnase 1 Solarbio Cat# D8071 DAPI Abcam Cat# ab104139 Citrate Sodium Buffer, pH 6.0 Solarbio Cat# C1010 Tris-EDTA buffer, pH 9.0 Elabscience Cat# E-IR-R104 Fetal Bovine Serum Gibco Cat# 10099141 Tween 20 Beyotime Cat# ST1727 Triton X-100 Beyotime Cat# ST797 RIPA buffer Beyotime Cat# P0013B Trizol reagent Invitrogen Cat# 15596026 D-Luciferin potassium salt Beyotime Cat# ST196 Lipofectamine 2000 reagent Invitrogen Cat# 11668019 Critical commercial assays RNeasy Mini Kit Qiagen Cat# 74104 PrimeScriptTM RT reagent Kit TAKARA Cat# RR037A CUT&Tag Kit YEASEN Cat# 12598ES12 True-Nuclear Transcription Factor Buffer Set Biolegend Cat# 424401 Mouse Collagen Type I ELISA Kit CUSABIO Cat# CSB-E08083m Mouse PBMC Isolation Kit IPHASE Cat# 071E100.11 Mouse CD8+ T cell Isolation Kit IPHASE Cat# 071E203.11 Deposited data RNA-seq of BV2 cells This paper GEO: GSE261941 Experimental models: Cell lines 4T1 Type Culture Collection of Chinese Academy of Sciences (Shanghai, China) N/A LLC1 Type Culture Collection of Chinese Academy of Sciences (Shanghai, China) N/A BV2 BeNa Culture Collection (Beijing, China) N/A 293FT BeNa Culture Collection (Beijing, China) N/A (Continued on next page) Cell Reports Medicine 5, 101533, May 21, 2024 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Organisms/strains BALB/C mice Guangdong Medical Laboratory Animal Center (Foshan, China) N/A C57BL/6 mice Guangdong Medical Laboratory Animal Center (Foshan, China) N/A Oligonucleotides Primer sequences are provided in the Table S1 This paper N/A Software and algorithms FlowJo BD Biosciences https://www.flowjo.com/ Prism, v8 GraphPad https://www.graphpad.com/ scientificsoftware/prism/ ImageJ NIH Image https://imagej.nih.gov/ij/index.html R (version 4.3.2) The R Project for Statistical Computing https://www.r-project.org/ R Studio PBC https://www.rstudio.com/ ComplexHeatmap (R package, version 2.16.0) N/A https://bioconductor.org/packages/ release/bioc/html/Complex Heatmap.html circlize (R package, version 0.4.15) N/A https://jokergoo.github.io/ circlize_book/book/ tidyverse (R package, version 2.0.0) N/A https://www.tidyverse.org/learn/ ggplot2 (R package, version 3.4.4) N/A https://ggplot2.tidyverse.org UMAP N/A https://arxiv.org/abs/1802.03426 ggvenn (R package, version 0.1.10) N/A https://CRAN.R-project.org/ package=ggvenn AnnotationDbi (R package, version 1.62.2) Bioconductor https://bioconductor.org/packages/ AnnotationDbi clusterProfiler (R package, version 4.8.3) Wu et al.39 https://yulab-smu.top/biomedical- knowledge-mining-book/ enrichplot (R package, version 1.20.3) Bioconductor https://bioconductor.org/packages/ release/bioc/html/enrichplot.html FastQC v0.11.9 N/A https://www.bioinformatics.babraham. ac.uk/projects/fastqc/ Trim galore v0.6.7 N/A https://www.bioinformatics.babraham. ac.uk/projects/trim_galore/ Hisat2 v2.2.1 Kim et al.41 http://daehwankimlab.github.io/hisat2/ DEseq2 (R package, version 1.40.2) Love et al.42 https://bioconductor.org/packages/ release/bioc/html/DESeq2.html infercnvpy v0.4.3 N/A https://infercnvpy.readthedocs.io/ en/latest/ Seurat (R package, version 5.0.1) N/A http://satijalab.org/seurat scanpy v1.9.3 N/A https://scanpy.readthedocs.io/ en/stable/ Other PVDF membrane Millipore Cat# IPVH00010 24-well plate Corning Cat# 3524 0.45 mm filter Sorfa Cat# 622120 70 mm cell filter ABCbio Cat# ABC60070 Transwell insert Corning Cat# 3422 e3 Cell Reports Medicine 5, 101533, May 21, 2024

    Cell Isolation:

    Article Title: Targeting the HSP47-collagen axis inhibits brain metastasis by reversing M2 microglial polarization and restoring anti-tumor immunity.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-HSP47 proteintech Cat# 10875-1-AP; RRID: AB_2301500 Anti-HSP47 Santa Cruz Cat# sc-5293; RRID: AB_627757 Anti-N-cadherin proteintech Cat# 22018-1-AP; RRID: AB_2813891 Anti-E-cadherin proteintech Cat# 20874-1-AP; RRID: AB_10697811 Anti-Snail proteintech Cat# 13099-1-AP; RRID: AB_2191756 Anti-Collagen I proteintech Cat# 14695-1-AP; RRID: AB_2082037 Anti-Collagen I proteintech Cat# 67288-1-Ig; RRID: AB_2882554 Anti-Collagen II proteintech Cat# 28459-1-AP; RRID: AB_2881147 Anti-Collagen III proteintech Cat# 22734-1-AP; RRID: AB_2879158 Anti-Collagen IV proteintech Cat# 19674-1-AP; RRID: AB_2878595 Anti-GFP-Tag Abclonal Cat# AE012; RRID: AB_2770402 Anti-Arg1 proteintech Cat# 16001-1-AP; RRID: AB_2289842 Anti-CD163 proteintech Cat# 16646-1-AP; RRID: AB_2756528 Anti-CD206 proteintech Cat# 18704-1-AP; RRID: AB_10597232 Anti- CD11c proteintech Cat# 17342-1-AP; RRID: AB_2129787 Anti-IBA1 proteintech Cat# 10904-1-AP; RRID: AB_2224377 Anti-CD8 Abcam Cat# ab217344; RRID: AB_2890649 Anti-CD8 Santa Cruz Cat# sc-7970; RRID: AB_627208 Anti-P65 CST Cat# 8242; RRID: AB_10859369 Anti-Phospho-P65 CST Cat# 3033; RRID: AB_331284 Anti-Tubulin Ray antibody Cat# RM2007; RRID: AB_2934267 CD11b-BV605 Biolegend Cat# 101257; RRID: AB_2565431 CD11c-APC Biolegend Cat# 117310; RRID: AB_313779 CD206-PE Biolegend Cat# 141706; RRID: AB_10895754 CD45-PE/cy7 Biolegend Cat# 103114; RRID: AB_312979 CD45-APC/cy7 BD Pharmingen Cat# 557659; RRID: AB_396774 CD3-PerCP/cy5.5 Biolegend Cat# 100218; RRID: AB_1595492 CD3-FITC Biolegend Cat# 100204; RRID: AB_312661 CD4-PE Biolegend Cat# 100408; RRID: AB_312693 CD8-APC/FireTM 750 Biolegend Cat# 100766; RRID: AB_2572113 CD8 PerCP/cy5.5 BD Pharmingen Cat# 551162; RRID: AB_394081 GZMB-BV421 Biolegend Cat# 396414; RRID: AB_2810603 TNF-a-BV650 Biolegend Cat# 506333; RRID: AB_2562450 IFN-g-BV510 Biolegend Cat# 505842; RRID: AB_2734494 Ki67-BV786 BD Pharmingen Cat# 563756; RRID: AB_2732007 CD69-AF700 Biolegend Cat# 104539; RRID: AB_2566304 CD25-BV605 BD Pharmingen Cat# 563061; RRID: AB_2737982 FOXP3-AF647 BD Pharmingen Cat# 560401; RRID: AB_1645201 Bacterial and virus strains EF1A-MCS-SV40-firefly_Luciferase-IRES-Puromycin Genechem https://www.genechem.com.cn/ pCLenti-EF1-Neo-CMV-HSP47-WPRE HanBio https://hanbio.net/ Tet-pLKO-neo YouBio http://www.youbio.cn/vector CMV-MCS-3flag-EF1-GFP-T2A-Blasticidin Genechem https://www.genechem.com.cn/ (Continued on next page) e1 Cell Reports Medicine 5, 101533, May 21, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER Biological samples Human primary breast cancer tissues and paired brain metastatic tissues Guangdong Provincial People’s Hospital of Southern Medical University (Guangzhou, China) N/A Human primary lung cancer tissues and paired brain metastatic tissues Guangdong Provincial People’s Hospital of Southern Medical University (Guangzhou, China) N/A Chemicals, peptides, and recombinant proteins Puromycin Thermo Fisher Scientific Cat# A1113803 Neomycin Solarbio Cat# IN0130 Blasticidin Selleck Cat# S7419 Col003 MedChemExpress Cat# HY-124817 TC-I 15 MedChemExpress Cat# HY-107588 InVivoMAb anti-mouse CD8a Selleck Cat# A2102 IgG2b isotype control-InVivo Selleck Cat# A2116 InVivoMAb anti-mouse PD-L1 (B7-H1) Bio X Cell Cat# BE0361; RRID: AB_2927503 PLX3397 MolDiets Cat# M18072603 Cultrex rCollagen I R&D Systems Cat# 3440-100-01 Collagenase 1 Worthington Cat# LS004197 ACK Lysis Buffer LEAGENE Cat# CS0001 Dnase 1 Solarbio Cat# D8071 DAPI Abcam Cat# ab104139 Citrate Sodium Buffer, pH 6.0 Solarbio Cat# C1010 Tris-EDTA buffer, pH 9.0 Elabscience Cat# E-IR-R104 Fetal Bovine Serum Gibco Cat# 10099141 Tween 20 Beyotime Cat# ST1727 Triton X-100 Beyotime Cat# ST797 RIPA buffer Beyotime Cat# P0013B Trizol reagent Invitrogen Cat# 15596026 D-Luciferin potassium salt Beyotime Cat# ST196 Lipofectamine 2000 reagent Invitrogen Cat# 11668019 Critical commercial assays RNeasy Mini Kit Qiagen Cat# 74104 PrimeScriptTM RT reagent Kit TAKARA Cat# RR037A CUT&Tag Kit YEASEN Cat# 12598ES12 True-Nuclear Transcription Factor Buffer Set Biolegend Cat# 424401 Mouse Collagen Type I ELISA Kit CUSABIO Cat# CSB-E08083m Mouse PBMC Isolation Kit IPHASE Cat# 071E100.11 Mouse CD8+ T cell Isolation Kit IPHASE Cat# 071E203.11 Deposited data RNA-seq of BV2 cells This paper GEO: GSE261941 Experimental models: Cell lines 4T1 Type Culture Collection of Chinese Academy of Sciences (Shanghai, China) N/A LLC1 Type Culture Collection of Chinese Academy of Sciences (Shanghai, China) N/A BV2 BeNa Culture Collection (Beijing, China) N/A 293FT BeNa Culture Collection (Beijing, China) N/A (Continued on next page) Cell Reports Medicine 5, 101533, May 21, 2024 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Organisms/strains BALB/C mice Guangdong Medical Laboratory Animal Center (Foshan, China) N/A C57BL/6 mice Guangdong Medical Laboratory Animal Center (Foshan, China) N/A Oligonucleotides Primer sequences are provided in the Table S1 This paper N/A Software and algorithms FlowJo BD Biosciences https://www.flowjo.com/ Prism, v8 GraphPad https://www.graphpad.com/ scientificsoftware/prism/ ImageJ NIH Image https://imagej.nih.gov/ij/index.html R (version 4.3.2) The R Project for Statistical Computing https://www.r-project.org/ R Studio PBC https://www.rstudio.com/ ComplexHeatmap (R package, version 2.16.0) N/A https://bioconductor.org/packages/ release/bioc/html/Complex Heatmap.html circlize (R package, version 0.4.15) N/A https://jokergoo.github.io/ circlize_book/book/ tidyverse (R package, version 2.0.0) N/A https://www.tidyverse.org/learn/ ggplot2 (R package, version 3.4.4) N/A https://ggplot2.tidyverse.org UMAP N/A https://arxiv.org/abs/1802.03426 ggvenn (R package, version 0.1.10) N/A https://CRAN.R-project.org/ package=ggvenn AnnotationDbi (R package, version 1.62.2) Bioconductor https://bioconductor.org/packages/ AnnotationDbi clusterProfiler (R package, version 4.8.3) Wu et al.39 https://yulab-smu.top/biomedical- knowledge-mining-book/ enrichplot (R package, version 1.20.3) Bioconductor https://bioconductor.org/packages/ release/bioc/html/enrichplot.html FastQC v0.11.9 N/A https://www.bioinformatics.babraham. ac.uk/projects/fastqc/ Trim galore v0.6.7 N/A https://www.bioinformatics.babraham. ac.uk/projects/trim_galore/ Hisat2 v2.2.1 Kim et al.41 http://daehwankimlab.github.io/hisat2/ DEseq2 (R package, version 1.40.2) Love et al.42 https://bioconductor.org/packages/ release/bioc/html/DESeq2.html infercnvpy v0.4.3 N/A https://infercnvpy.readthedocs.io/ en/latest/ Seurat (R package, version 5.0.1) N/A http://satijalab.org/seurat scanpy v1.9.3 N/A https://scanpy.readthedocs.io/ en/stable/ Other PVDF membrane Millipore Cat# IPVH00010 24-well plate Corning Cat# 3524 0.45 mm filter Sorfa Cat# 622120 70 mm cell filter ABCbio Cat# ABC60070 Transwell insert Corning Cat# 3422 e3 Cell Reports Medicine 5, 101533, May 21, 2024

    RNA Sequencing:

    Article Title: Targeting the HSP47-collagen axis inhibits brain metastasis by reversing M2 microglial polarization and restoring anti-tumor immunity.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-HSP47 proteintech Cat# 10875-1-AP; RRID: AB_2301500 Anti-HSP47 Santa Cruz Cat# sc-5293; RRID: AB_627757 Anti-N-cadherin proteintech Cat# 22018-1-AP; RRID: AB_2813891 Anti-E-cadherin proteintech Cat# 20874-1-AP; RRID: AB_10697811 Anti-Snail proteintech Cat# 13099-1-AP; RRID: AB_2191756 Anti-Collagen I proteintech Cat# 14695-1-AP; RRID: AB_2082037 Anti-Collagen I proteintech Cat# 67288-1-Ig; RRID: AB_2882554 Anti-Collagen II proteintech Cat# 28459-1-AP; RRID: AB_2881147 Anti-Collagen III proteintech Cat# 22734-1-AP; RRID: AB_2879158 Anti-Collagen IV proteintech Cat# 19674-1-AP; RRID: AB_2878595 Anti-GFP-Tag Abclonal Cat# AE012; RRID: AB_2770402 Anti-Arg1 proteintech Cat# 16001-1-AP; RRID: AB_2289842 Anti-CD163 proteintech Cat# 16646-1-AP; RRID: AB_2756528 Anti-CD206 proteintech Cat# 18704-1-AP; RRID: AB_10597232 Anti- CD11c proteintech Cat# 17342-1-AP; RRID: AB_2129787 Anti-IBA1 proteintech Cat# 10904-1-AP; RRID: AB_2224377 Anti-CD8 Abcam Cat# ab217344; RRID: AB_2890649 Anti-CD8 Santa Cruz Cat# sc-7970; RRID: AB_627208 Anti-P65 CST Cat# 8242; RRID: AB_10859369 Anti-Phospho-P65 CST Cat# 3033; RRID: AB_331284 Anti-Tubulin Ray antibody Cat# RM2007; RRID: AB_2934267 CD11b-BV605 Biolegend Cat# 101257; RRID: AB_2565431 CD11c-APC Biolegend Cat# 117310; RRID: AB_313779 CD206-PE Biolegend Cat# 141706; RRID: AB_10895754 CD45-PE/cy7 Biolegend Cat# 103114; RRID: AB_312979 CD45-APC/cy7 BD Pharmingen Cat# 557659; RRID: AB_396774 CD3-PerCP/cy5.5 Biolegend Cat# 100218; RRID: AB_1595492 CD3-FITC Biolegend Cat# 100204; RRID: AB_312661 CD4-PE Biolegend Cat# 100408; RRID: AB_312693 CD8-APC/FireTM 750 Biolegend Cat# 100766; RRID: AB_2572113 CD8 PerCP/cy5.5 BD Pharmingen Cat# 551162; RRID: AB_394081 GZMB-BV421 Biolegend Cat# 396414; RRID: AB_2810603 TNF-a-BV650 Biolegend Cat# 506333; RRID: AB_2562450 IFN-g-BV510 Biolegend Cat# 505842; RRID: AB_2734494 Ki67-BV786 BD Pharmingen Cat# 563756; RRID: AB_2732007 CD69-AF700 Biolegend Cat# 104539; RRID: AB_2566304 CD25-BV605 BD Pharmingen Cat# 563061; RRID: AB_2737982 FOXP3-AF647 BD Pharmingen Cat# 560401; RRID: AB_1645201 Bacterial and virus strains EF1A-MCS-SV40-firefly_Luciferase-IRES-Puromycin Genechem https://www.genechem.com.cn/ pCLenti-EF1-Neo-CMV-HSP47-WPRE HanBio https://hanbio.net/ Tet-pLKO-neo YouBio http://www.youbio.cn/vector CMV-MCS-3flag-EF1-GFP-T2A-Blasticidin Genechem https://www.genechem.com.cn/ (Continued on next page) e1 Cell Reports Medicine 5, 101533, May 21, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER Biological samples Human primary breast cancer tissues and paired brain metastatic tissues Guangdong Provincial People’s Hospital of Southern Medical University (Guangzhou, China) N/A Human primary lung cancer tissues and paired brain metastatic tissues Guangdong Provincial People’s Hospital of Southern Medical University (Guangzhou, China) N/A Chemicals, peptides, and recombinant proteins Puromycin Thermo Fisher Scientific Cat# A1113803 Neomycin Solarbio Cat# IN0130 Blasticidin Selleck Cat# S7419 Col003 MedChemExpress Cat# HY-124817 TC-I 15 MedChemExpress Cat# HY-107588 InVivoMAb anti-mouse CD8a Selleck Cat# A2102 IgG2b isotype control-InVivo Selleck Cat# A2116 InVivoMAb anti-mouse PD-L1 (B7-H1) Bio X Cell Cat# BE0361; RRID: AB_2927503 PLX3397 MolDiets Cat# M18072603 Cultrex rCollagen I R&D Systems Cat# 3440-100-01 Collagenase 1 Worthington Cat# LS004197 ACK Lysis Buffer LEAGENE Cat# CS0001 Dnase 1 Solarbio Cat# D8071 DAPI Abcam Cat# ab104139 Citrate Sodium Buffer, pH 6.0 Solarbio Cat# C1010 Tris-EDTA buffer, pH 9.0 Elabscience Cat# E-IR-R104 Fetal Bovine Serum Gibco Cat# 10099141 Tween 20 Beyotime Cat# ST1727 Triton X-100 Beyotime Cat# ST797 RIPA buffer Beyotime Cat# P0013B Trizol reagent Invitrogen Cat# 15596026 D-Luciferin potassium salt Beyotime Cat# ST196 Lipofectamine 2000 reagent Invitrogen Cat# 11668019 Critical commercial assays RNeasy Mini Kit Qiagen Cat# 74104 PrimeScriptTM RT reagent Kit TAKARA Cat# RR037A CUT&Tag Kit YEASEN Cat# 12598ES12 True-Nuclear Transcription Factor Buffer Set Biolegend Cat# 424401 Mouse Collagen Type I ELISA Kit CUSABIO Cat# CSB-E08083m Mouse PBMC Isolation Kit IPHASE Cat# 071E100.11 Mouse CD8+ T cell Isolation Kit IPHASE Cat# 071E203.11 Deposited data RNA-seq of BV2 cells This paper GEO: GSE261941 Experimental models: Cell lines 4T1 Type Culture Collection of Chinese Academy of Sciences (Shanghai, China) N/A LLC1 Type Culture Collection of Chinese Academy of Sciences (Shanghai, China) N/A BV2 BeNa Culture Collection (Beijing, China) N/A 293FT BeNa Culture Collection (Beijing, China) N/A (Continued on next page) Cell Reports Medicine 5, 101533, May 21, 2024 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Organisms/strains BALB/C mice Guangdong Medical Laboratory Animal Center (Foshan, China) N/A C57BL/6 mice Guangdong Medical Laboratory Animal Center (Foshan, China) N/A Oligonucleotides Primer sequences are provided in the Table S1 This paper N/A Software and algorithms FlowJo BD Biosciences https://www.flowjo.com/ Prism, v8 GraphPad https://www.graphpad.com/ scientificsoftware/prism/ ImageJ NIH Image https://imagej.nih.gov/ij/index.html R (version 4.3.2) The R Project for Statistical Computing https://www.r-project.org/ R Studio PBC https://www.rstudio.com/ ComplexHeatmap (R package, version 2.16.0) N/A https://bioconductor.org/packages/ release/bioc/html/Complex Heatmap.html circlize (R package, version 0.4.15) N/A https://jokergoo.github.io/ circlize_book/book/ tidyverse (R package, version 2.0.0) N/A https://www.tidyverse.org/learn/ ggplot2 (R package, version 3.4.4) N/A https://ggplot2.tidyverse.org UMAP N/A https://arxiv.org/abs/1802.03426 ggvenn (R package, version 0.1.10) N/A https://CRAN.R-project.org/ package=ggvenn AnnotationDbi (R package, version 1.62.2) Bioconductor https://bioconductor.org/packages/ AnnotationDbi clusterProfiler (R package, version 4.8.3) Wu et al.39 https://yulab-smu.top/biomedical- knowledge-mining-book/ enrichplot (R package, version 1.20.3) Bioconductor https://bioconductor.org/packages/ release/bioc/html/enrichplot.html FastQC v0.11.9 N/A https://www.bioinformatics.babraham. ac.uk/projects/fastqc/ Trim galore v0.6.7 N/A https://www.bioinformatics.babraham. ac.uk/projects/trim_galore/ Hisat2 v2.2.1 Kim et al.41 http://daehwankimlab.github.io/hisat2/ DEseq2 (R package, version 1.40.2) Love et al.42 https://bioconductor.org/packages/ release/bioc/html/DESeq2.html infercnvpy v0.4.3 N/A https://infercnvpy.readthedocs.io/ en/latest/ Seurat (R package, version 5.0.1) N/A http://satijalab.org/seurat scanpy v1.9.3 N/A https://scanpy.readthedocs.io/ en/stable/ Other PVDF membrane Millipore Cat# IPVH00010 24-well plate Corning Cat# 3524 0.45 mm filter Sorfa Cat# 622120 70 mm cell filter ABCbio Cat# ABC60070 Transwell insert Corning Cat# 3422 e3 Cell Reports Medicine 5, 101533, May 21, 2024



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    Image Search Results


    Vincristine affects trabecular, cortical bone and increases a plasma marker associated with bone resorption (CTX-1). A Quantification of trabecular bone volume fraction (Tb.BV/TV; %), B trabecular thickness (Tb.Th; μm), C trabecular number (Tb.N; μm), D trabecular spacing (Tb.Sp; μm), E connectivity density (Conn.Dn; 1/mm3), F cortical thickness (Ct.Th: mm), G cortical area (Ct.Ar; mm2), H fraction of cortical bone relative to total bone (Ct.Ar/Tt.Ar; %) in the femur of mice. I resistance to force (Polar MOI; J:mm4). J Plasma C-terminal telopeptide of type I collagen (CTX-1; pg/mL). K Representative 3-D images of trabecular bone and cortical bone. Data reported as means ± SD. Different letters denote significant differences: p <0.05. Control ( n =5); Vincristine ( n =5)

    Journal: BMC Cancer

    Article Title: Vincristine impairs musculoskeletal development in pediatric mice

    doi: 10.1186/s12885-025-15262-x

    Figure Lengend Snippet: Vincristine affects trabecular, cortical bone and increases a plasma marker associated with bone resorption (CTX-1). A Quantification of trabecular bone volume fraction (Tb.BV/TV; %), B trabecular thickness (Tb.Th; μm), C trabecular number (Tb.N; μm), D trabecular spacing (Tb.Sp; μm), E connectivity density (Conn.Dn; 1/mm3), F cortical thickness (Ct.Th: mm), G cortical area (Ct.Ar; mm2), H fraction of cortical bone relative to total bone (Ct.Ar/Tt.Ar; %) in the femur of mice. I resistance to force (Polar MOI; J:mm4). J Plasma C-terminal telopeptide of type I collagen (CTX-1; pg/mL). K Representative 3-D images of trabecular bone and cortical bone. Data reported as means ± SD. Different letters denote significant differences: p <0.05. Control ( n =5); Vincristine ( n =5)

    Article Snippet: The levels of circulating C-terminal telopeptide of type I collagen (CTX-1) were measured in the plasma from EDTA-treated blood collected from vehicle- and vincristine-treated mice using a specific ELISA kit (NB030329; Novus Biologicals Mouse CTX-1 ELISA Kit, Centennial, USA) according to the manufacturer’s protocol.

    Techniques: Clinical Proteomics, Marker, Control